NGS و بیوانفورماتیک

Single-cell RNA-seq Data Analysis

Progress from raw scRNA-seq reads to a cellular count matrix and Seurat-based clustering, with focused introductions to cell communication and circRNA.

Milad Eidi 10 lessons
Single-cell RNA-seq Data Analysis
3,890,000 Toman
Login to Purchase

30-Day Money-Back Guarantee

What You'll Learn

  • Understand scRNA-seq protocols and move from FASTQ files to a cellular count matrix
  • Cluster cells with Seurat and extract cluster-level count data
  • Explore cell communication and the supplementary circRNA module

About This Course

Learn the core path for analyzing gene expression at single-cell resolution. Beginning with scRNA-seq protocols, the course moves through count-matrix construction, cell clustering, and extraction of data for selected clusters.

The curriculum also introduces cell-to-cell communication and includes a supplementary single-cell session plus circRNA identification, quantification, and differential-expression material. A dedicated course is available for deeper study of cell communication.

Core single-cell topics

  • From raw reads to a matrixUnderstand scRNA-seq protocols and the steps from FASTQ files to a cellular count matrix.
  • Cell clusteringUse Seurat concepts to cluster cells and extract count matrices for selected groups.
  • Cell-to-cell communicationSee where communication analysis fits within a single-cell workflow and discover the dedicated follow-on course.

Supplementary module: circRNA

  • Identification and differential expressionIntroduce circular RNA, identify and quantify circRNAs through two workflows, and evaluate differential expression.

Who is this course for?

  • Students and researchers ready to begin single-cell RNA-seq analysis.
  • Expression analysts who want to expand their skills to single-cell datasets.

Related learning

Recommended preparation

  • Familiarity with NGS, gene expression, and data matrices is recommended.
  • Basic R skills and experience with count data will help you follow the examples.

Curriculum 2 sections

  • جلسه اول آنالیز Single Cell
  • جلسه دوم آنالیز Single Cell
  • جلسه سوم آنالیز Single Cell
  • جلسه چهارم آنالیز Single Cell
  • جلسات اسکایپ
  • جلسه آپدیت - بخش Single Cell RNA Seq

  • بخش اول Circulating RNA
  • بخش دوم Circulating RNA
  • بخش سوم Circulating RNA
  • بخش چهارم Circulating RNA

Student Reviews

No reviews yet. Be the first to review this course!

Instructor
Milad Eidi
Milad Eidi
Bioinformatics Expert

Experienced instructor in bioinformatics and computational biology.

Course Details
  • 2 sections
  • 10 lessons
  • Full lifetime access