Develop a connected view of the molecular genetics workflow with Sanger sequencing at its center. The course begins with extraction, primer design, and PCR, then moves into sequence analysis and variant interpretation.
Guided examples cover both germline and somatic contexts, while practical troubleshooting helps you recognize common problems in PCR and sequence analysis.
Core topics
- Experimental preparationReview extraction considerations, primer design, and PCR execution within a molecular workflow.
- Sanger sequence analysisExamine training examples, evaluate sequences, and follow the process of identifying variants.
- Interpretation and troubleshootingExplore the ACMG and AMP frameworks introduced in the course and investigate common laboratory and analysis challenges.
Who is this course for?
- Genetics students and professionals interested in molecular laboratory work.
- Laboratory staff and researchers seeking a more structured Sanger workflow.
